Overview

  • Product nameAnti-CD4 antibody [OX-35]See all CD4 primary antibodies ...
  • Description
    Mouse monoclonal [OX-35] to CD4
  • Tested applicationsICC, Flow Cyt, IHC-Fr more details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    MLR generated rat T helper lymphocytes.

  • EpitopeThis antibody recognises a different epitope on the CD4 molecule than the W3/25 monoclonal (eg ab33779 and ab33783).

Properties

Applications

Our Abpromise guarantee covers the use of ab33775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

References for Anti-CD4 antibody [OX-35] (ab33775)

This product has been referenced in:
  • Thampi P  et al. Pathological effects of processed bovine pericardial scaffolds--a comparative in vivo evaluation. Artif Organs 37:600-5 (2013). IHC ; Rat . Read more (PubMed: 23452255) »
  • Pohlers D  et al. Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on NF-kappaB binding activity and TNF-alpha secretion of T cells. Arthritis Res 4:184-9 (2002). Read more (PubMed: 12010568) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. ab31630, can be used in IHC-P ab8536, can be used in IHC-P ab65865; IHC-P is yet not tested we are unable to confirm it. Can we suggest alternatives as ab34364, ab87951 and ab101500? ab33775; IHC-P is not a ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"