Overview

  • Product nameAnti-CD4 antibody [OX-35]
    See all CD4 primary antibodies
  • Description
    Mouse monoclonal [OX-35] to CD4
  • Tested applicationsICC, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    MLR generated rat T helper lymphocytes.

  • EpitopeThis antibody recognises a different epitope on the CD4 molecule than the W3/25 monoclonal (eg ab33779 and ab33783).
  • General notesAb18414-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab33775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

References for Anti-CD4 antibody [OX-35] (ab33775)

This product has been referenced in:
  • Thampi P  et al. Pathological effects of processed bovine pericardial scaffolds--a comparative in vivo evaluation. Artif Organs 37:600-5 (2013). IHC ; Rat . Read more (PubMed: 23452255) »
  • Pohlers D  et al. Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on NF-kappaB binding activity and TNF-alpha secretion of T cells. Arthritis Res 4:184-9 (2002). Read more (PubMed: 12010568) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us. ab31630, can be used in IHC-P ab8536, can be used in IHC-P ab65865; IHC-P is yet not tested we are unable to confirm it. Can we suggest alternatives as ab34364, ab87951 and ab101500? ab33775; IHC-P is not a ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"