Anti-CD4 antibody [SP35], prediluted (ab101530)


  • Product nameAnti-CD4 antibody [SP35], prediluted
    See all CD4 primary antibodies
  • Description
    Rabbit monoclonal [SP35] to CD4, prediluted
  • Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    A synthetic peptide corresponding to internal region of Human CD4.

  • Positive control
    • Human Tonsil, Human T cell lymphoma



Our Abpromise guarantee covers the use of ab101530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.


  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [SP35], prediluted images

  • Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody, prediluted, ab101530 in Jurkats cells (green) compared to negative control of rabbit IgG (blue)

  • Staining of Human CD4 in a Formalin/PFA-fixed paraffin-embedded section of Human T cell lymphoma using ab101530 at a dilution of 1/1.

References for Anti-CD4 antibody [SP35], prediluted (ab101530)

ab101530 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 40% · Temperature: 20°C
Sample Human Tissue sections (human tonsil)
Specification human tonsil
Permeabilization Yes - saponin
Fixative Paraformaldehyde

Mr. Jan Misiak

Verified customer

Submitted May 13 2014

Thank you for your enquiry.

I have checked these product numbers. I am sorry to confirm that ab4098 is discontinued and apologize for the inconvenience.

ab4098 is an antibody for detection of Nucleophosmin. However, ab101530 is an...

Read More

Thank you very much for your phone call.

The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

Anti CD3 : ab...

Read More