Overview

  • Product nameAnti-CD4 antibody [YNB46.1.8]See all CD4 primary antibodies ...
  • Description
    Rat monoclonal [YNB46.1.8] to CD4
  • Tested applicationsIHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): Rat cells transfected with human CD4

  • General notes


    This antibody blocks syncytia formation of U937 cells infected with HIV.

Properties

Applications

Our Abpromise guarantee covers the use of ab34276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/200. Use 10µl of this concentration to label 106 cells in 100µl.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 antibody [YNB46.1.8] images

  • ab34276 staining Human peripheral blood lymphocytes by Flow Cytometry.

References for Anti-CD4 antibody [YNB46.1.8] (ab34276)

This product has been referenced in:
  • Fujii T  et al. SLURP-1, an endogenous a7 nicotinic acetylcholine receptor allosteric ligand, is expressed in CD205(+) dendritic cells in human tonsils and potentiates lymphocytic cholinergic activity. J Neuroimmunol 267:43-9 (2014). Human . Read more (PubMed: 24365495) »
  • Zhu J  et al. Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisition. Nat Med 15:886-92 (2009). IHC-Fr ; Human . Read more (PubMed: 19648930) »

See all 3 Publications for this product

Product Wall

This is a follow up regarding your recently published Abreview of ab31962, an anti-Wnt11 antibody, to which you have assigned a 'Poor' rating. From your image it appears there is weak staining of the cell periphery, as would be expected for CD4. Howeve...

Read More
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Acetone
Permeabilization Yes - 0.1% triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"