Overview

  • Product name
    Anti-CD4 antibody [YNB46.1.8]
    See all CD4 primary antibodies
  • Description
    Rat monoclonal [YNB46.1.8] to CD4
  • Tested applications
    Suitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): Rat cells transfected with human CD4

  • General notes


    This antibody blocks syncytia formation of U937 cells infected with HIV.

Properties

Applications

Our Abpromise guarantee covers the use of ab34276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/100 - 1/200. Use 10µl of this concentration to label 106 cells in 100µl.



ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similarities
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localization
    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Images

  • ab34276 staining Human peripheral blood lymphocytes by Flow Cytometry.
  • Immunohistochemistry (Frozen sections) analysis of human palatine tonsil tissue labelling CD4 (red) with ab34276 at 1/50. A Cy3-conjugated donkey anti-rat IgG was used as the secondary antibody. Green - SLURP-1, Blue - Nuclei (DAPI).

References

This product has been referenced in:
  • Su S  et al. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer. Cell Res 27:461-482 (2017). IHC ; Human . Read more (PubMed: 28290464) »
  • Fujii T  et al. SLURP-1, an endogenous a7 nicotinic acetylcholine receptor allosteric ligand, is expressed in CD205(+) dendritic cells in human tonsils and potentiates lymphocytic cholinergic activity. J Neuroimmunol 267:43-9 (2014). IHC-Fr ; Human . Read more (PubMed: 24365495) »

See all 4 Publications for this product

Customer reviews and Q&As

This is a follow up regarding your recently published Abreview of ab31962, an anti-Wnt11 antibody, to which you have assigned a 'Poor' rating. From your image it appears there is weak staining of the cell periphery, as would be expected for CD4. Howeve...

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Acetone
Permeabilization
Yes - 0.1% triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

Submitted Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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