Tissue/ cell preparation (Human): Rat cells transfected with human CD4
This antibody blocks syncytia formation of U937 cells infected with HIV.
Our Abpromise guarantee covers the use of ab34276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||1/100 - 1/200. Use 10µl of this concentration to label 106 cells in 100µl.
ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunohistochemistry (Frozen sections) analysis of human palatine tonsil tissue labelling CD4 (red) with ab34276 at 1/50. A Cy3-conjugated donkey anti-rat IgG was used as the secondary antibody. Green - SLURP-1, Blue - Nuclei (DAPI).