Dual color: FITC + PE Anti-CD4 + CD8 antibody [EDU-2 + 733] (ab1232)
Key features and details
- Dual color: FITC + PE Mouse monoclonal [EDU-2 + 733] to CD4 + CD8
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Dual color: FITC + PE
Overview
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Product name
Dual color: FITC + PE Anti-CD4 + CD8 antibody [EDU-2 + 733]
See all CD4 + CD8 primary antibodies -
Description
Dual color: FITC + PE Mouse monoclonal [EDU-2 + 733] to CD4 + CD8 -
Host species
Mouse -
Conjugation
Dual color: FITC + PE -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. Stimulated human leukocytes
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General notes
This cocktail is mix of: CD4 = Clone:EDU-2, Isotype:IgG2a, Labeled with FITC and CD8 = Clone:733, Isotype:IgG1, Labeled with PE.This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EDU-2 + 733 -
Myeloma
unknown -
Light chain type
unknown -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Notes |
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Percentage count of T suppressor/cytotoxic lymphocytes (CD8+).
Control autoimmune pathologies, immunodeficiencies and immunosuppressivetherapies.
Studies of HIV (Human Immunodeficiency Virus) infection.
CD4(FITC) / CD8 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD4(FITC) / CD8 (PE) is sufficient for labelling 1x106 cells.
Target
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Relevance
CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. -
Cellular localization
Membrane; single pass type I membrane protein. -
Database links
- Entrez Gene: 920 Human
- Entrez Gene: 925 Human
- Entrez Gene: 926 Human
- Omim: 186730 Human
- Omim: 186910 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- SwissProt: P01732 Human
see all -
Alternative names
- CD4 antigen antibody
- CD4 molecule antibody
- CD4 mut antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab1232 has been referenced in 1 publication.
- Wang D et al. Genome-wide DNA methylation pattern in a mouse model reveals two novel genes associated with Staphylococcus aureus mastitis. Asian-Australas J Anim Sci 33:203-211 (2020). PubMed: 31010979