Anti-CD4 [RFT-4g] antibody (Allophycocyanin) (ab25617)

Overview

  • Product nameAnti-CD4 [RFT-4g] antibody (Allophycocyanin)See all CD4 primary antibodies ...
  • Description
    Mouse monoclonal [RFT-4g] to CD4 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab25617 recognises human CD4.
  • Tested applicationsFlow Cyt, IP, IHC-Fr more details
  • Species reactivity
    Reacts with: Rat, Human, Cynomolgus Monkey, Rhesus monkey, Marmoset (common)
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 10µl for 106 cells.
IP Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 Antigen antibody
    • CD4 molecule antibody
    • CD4 Receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T Cell Antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T Cell Surface Antigen T4/Leu3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T Cell Surface Glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 [RFT-4g] antibody (Allophycocyanin) images

  • ab25617 staining CD4 in Rat blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C.
    Gating Strategy: Live lymphocytes.

    See Abreview

  • ab25617 staining CD4 in Marmoset (common) blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C.

    See Abreview

  • ab25617 staining CD4 in Rhesus monkey blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C.
    Gating Strategy: Live lymphocytes.

    See Abreview

  • Flow cytometry analysis of monkey blood, staining CD4 with ab25617.

    Red blood cells were lysed and samples were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 2% FCS in PBS) for 1 hour at 4°C.

    Gating Strategy: Live cells

    See Abreview

References for Anti-CD4 [RFT-4g] antibody (Allophycocyanin) (ab25617)

ab25617 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Rhesus monkey Cell (blood)
Specification blood
Gating Strategy alive lymphocytes
Preparation Cell harvesting/tissue preparation method: ACK lysis of red blood cells
Sample buffer: PBS/BSA 0.5%
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Submitted Oct 31 2013

Application Flow Cytometry
Permeabilization No
Sample Marmoset (common) Cell (blood)
Specification blood
Preparation Cell harvesting/tissue preparation method: ACK lysis of red blood cells
Sample buffer: PBS/BSA 0.5%
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Submitted Oct 31 2013

Application Flow Cytometry
Permeabilization No
Sample Rat Cell (splenocytes)
Specification splenocytes
Gating Strategy alive lymphocytes
Preparation Cell harvesting/tissue preparation method: ACK lysis of red blood cells
Sample buffer: PBS/BSA 0.5%
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Submitted Oct 31 2013

Application Flow Cytometry
Permeabilization No
Sample Mouse Cell (from lymph nodes)
Specification from lymph nodes
Gating Strategy lymphocytes
Preparation Cell harvesting/tissue preparation method: mechanical disruption
Sample buffer: PBS/BSA 0.5%
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Submitted Oct 31 2013

Application Flow Cytometry
Permeabilization No
Sample Cynomolgus Monkey Cell (blood)
Specification blood
Gating Strategy alive lymphocytes
Preparation Cell harvesting/tissue preparation method: ACK lysis of red blood cells
Sample buffer: PBS/BSA 0.5%
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Submitted Oct 31 2013

Application Flow Cytometry
Permeabilization No
Sample Human Cell (PBMC)
Specification PBMC
Gating Strategy lymphocytes
Preparation Cell harvesting/tissue preparation method: after ficoll
Sample buffer: PBS/BSA 0.5%
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Submitted Oct 31 2013

Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy alive cells
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Submitted Jan 10 2013

Application Flow Cytometry
Sample Monkey Cell (cells from blood)
Specification cells from blood
Preparation Cell harvesting/tissue preparation method: erythrocyte lysis
Sample buffer: PBS+2%FCS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
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Submitted Jan 04 2013

Application Flow Cytometry
Sample Mouse Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: isolated from spleen
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive cells
Username

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Submitted Jan 02 2013

Application Flow Cytometry
Sample Cynomolgus Monkey Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

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Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"