RabMAb

Anti-CD4 [SP35] antibody, prediluted (ab101530)

Overview

  • Product nameAnti-CD4 [SP35] antibody, predilutedSee all CD4 primary antibodies ...
  • Description
    Rabbit monoclonal [SP35] to CD4, prediluted
  • Tested applicationsFlow Cyt, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    A synthetic peptide corresponding to internal region of Human CD4.

  • Positive control
    • Human Tonsil, Human T cell lymphoma

Properties

Applications

Our Abpromise guarantee covers the use of ab101530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Antigen retrieval is recommended: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • FunctionAccessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
  • Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
  • Cellular localizationCell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Target information above from: UniProt accession P01730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CD 4 antibody
    • CD4 (L3T4) antibody
    • CD4 antibody
    • CD4 antibody
    • CD4 antigen (p55) antibody
    • CD4 antigen antibody
    • CD4 molecule antibody
    • CD4 receptor antibody
    • CD4+ Lymphocyte deficiency, included antibody
    • CD4_HUMAN antibody
    • CD4mut antibody
    • L3T4 antibody
    • Leu3 antibody
    • Ly-4 antibody
    • Lymphocyte antigen CD4 antibody
    • MGC165891 antibody
    • OTTHUMP00000238897 antibody
    • p55 antibody
    • T cell antigen T4 antibody
    • T cell antigen T4/LEU3 antibody
    • T cell differentiation antigen L3T4 antibody
    • T cell OKT4 deficiency, included antibody
    • T cell surface antigen T4/Leu 3 antibody
    • T cell surface antigen T4/Leu3 antibody
    • T cell surface glycoprotein CD4 antibody
    • T-cell surface antigen T4/Leu-3 antibody
    • T-cell surface glycoprotein CD4 antibody
    • W3/25 antibody
    • W3/25 antigen antibody
    see all

Anti-CD4 [SP35] antibody, prediluted images

  • Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody, prediluted, ab101530 in Jurkats cells (green) compared to negative control of rabbit IgG (blue)

  • Staining of Human CD4 in a Formalin/PFA-fixed paraffin-embedded section of Human T cell lymphoma using ab101530 at a dilution of 1/1.

References for Anti-CD4 [SP35] antibody, prediluted (ab101530)

ab101530 has not yet been referenced specifically in any publications.

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