Recombinant Anti-CD4 antibody [SP35], prediluted (ab101530)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP35] to CD4, prediluted
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD4 antibody [SP35], prediluted
See all CD4 primary antibodies -
Description
Rabbit monoclonal [SP35] to CD4, prediluted -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- Human Tonsil, Human T cell lymphoma
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP35 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD4 antibody [SP35] (ab213215)
- Anti-CD4 antibody [SP35] - BSA and Azide free (ab238798)
- Alexa Fluor® 488 Anti-CD4 antibody [SP35] (ab309931)
- Alexa Fluor® 647 Anti-CD4 antibody [SP35] (ab310298)
- Alexa Fluor® 594 Anti-CD4 antibody [SP35] (ab310760)
- Alexa Fluor® 555 Anti-CD4 antibody [SP35] (ab312287)
- Alexa Fluor® 568 Anti-CD4 antibody [SP35] (ab312782)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
Use at an assay dependent concentration.
Antigen retrieval is recommended: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. Antigen retrieval is recommended: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab101530 has been referenced in 3 publications.
- Gong TJ et al. Case Report: Pulmonary Metastases From Epithelioid Sarcoma in Extremity Favourably Responding to Immunotherapy With Camrelizumab. Front Oncol 11:728437 (2021). PubMed: 34692503
- Ravanidis S et al. Subcutaneous Transplantation of Neural Precursor Cells in Experimental Autoimmune Encephalomyelitis Reduces Chemotactic Signals in the Central Nervous System. Stem Cells Transl Med 4:1450-62 (2015). PubMed: 26511651
- Wick DA et al. Surveillance of the tumor mutanome by T cells during progression from primary to recurrent ovarian cancer. Clin Cancer Res 20:1125-34 (2014). PubMed: 24323902