Specificity was checked by ELISA and flow cytometry using BHK cells transfected with mouse CD40. It does not react with normal mouse Ig or with human Ig1 and will stain most mature mouse B cells. It dos not cross react with mouse T cells.
Clone 3/23 is a powerful activator of normal B cells especially in the prescence of IL4.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10ul of the suggested working dilution to label 10E6 cells in 100ul.
Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for TNFSF5/CD40LG.
B-cells and in primary carcinomas.
Involvement in disease
Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.