• Product nameAnti-CD40 antibody
    See all CD40 primary antibodies
  • Description
    Rabbit polyclonal to CD40
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human CD40.

  • Positive control
    • This antibody gave a positive signal in Human Spleen and Thymus tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.



Our Abpromise guarantee covers the use of ab113701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 31 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • FunctionReceptor for TNFSF5/CD40LG.
  • Tissue specificityB-cells and in primary carcinomas.
  • Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody images

  • All lanes : Anti-CD40 antibody (ab113701) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,41 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • IHC image of CD40 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113701, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD40 antibody (ab113701)

ab113701 has not yet been referenced specifically in any publications.

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