Overview

  • Product nameAnti-CD40 antibody
    See all CD40 primary antibodies
  • Description
    Rabbit polyclonal to CD40
  • SpecificityThis antibody detects endogenous levels of total CD40 protein.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the C-terminal of CD40 (Human)

  • Positive control
    • COS7 cell extract (Western blot). Human breast carcinoma (IHC-P). IF/ICC: RAW246.7 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab58391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionReceptor for TNFSF5/CD40LG.
  • Tissue specificityB-cells and in primary carcinomas.
  • Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody images

  • All lanes : Anti-CD40 antibody (ab58391) at 1/500 dilution

    Lane 1 : COS7 cell extract with no immunizing peptide
    Lane 2 : COS7 cell extract with immunizing peptide


    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • ab58391, at 1/50 diltuion, staining human CD40 in breast carcinoma tissue, by immunohistochemistry, in the presence or absence of the immunizing peptide. Paraffin embedded tissue.
  • ICC/IF image of ab58391 stained RAW246.7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58391, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CD40 antibody (ab58391)

This product has been referenced in:
  • Cowan RW  et al. T cells stimulate catabolic gene expression by the stromal cells from giant cell tumor of bone. Biochem Biophys Res Commun 419:719-23 (2012). IHC . Read more (PubMed: 22386994) »
  • Manczak M  et al. Neutralization of granulocyte macrophage colony-stimulating factor decreases amyloid beta 1-42 and suppresses microglial activity in a transgenic mouse model of Alzheimer's disease. Hum Mol Genet 18:3876-93 (2009). IHC-Fr ; Mouse . Read more (PubMed: 19617638) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (fibroblast)
Specification fibroblast
Permeabilization No
Fixative Paraformaldehyde
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Verified customer

Submitted Feb 27 2014

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with ab58612. The order number for this replacement is xxx.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"