The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionReceptor for TNFSF5/CD40LG.
Tissue specificityB-cells and in primary carcinomas.
Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
CD40 is a transmembrane glycoprotein that runs at 48 to 50 kDa on SDS page gels.
References for Anti-CD40 antibody [B-B20] (ab47021)
This product has been referenced in:
Pan W et al. Resveratrol Protects against TNF-a-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK. PLoS One11:e0147034 (2016).
Read more (PubMed: 26799794) »
Holl V et al. Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes. J Virol84:4172-82 (2010).
Read more (PubMed: 20147388) »