Overview

  • Product nameAnti-CD40 antibody [B-B20]
    See all CD40 primary antibodies
  • Description
    Mouse monoclonal [B-B20] to CD40
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation of Raji cell line (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab47021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for TNFSF5/CD40LG.
  • Tissue specificityB-cells and in primary carcinomas.
  • Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody [B-B20] images

  • A typical staining pattern with the B-B20 monoclonal antibody of lymphocytesFACS analysis of CD40 expression in Human lymphocytes using ab47021.
  • All lanes : Anti-CD40 antibody [B-B20] (ab47021) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 30 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CD40 antibody [B-B20] (ab47021)

This product has been referenced in:
  • Holl V  et al. Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes. J Virol 84:4172-82 (2010). Flow Cyt ; Human . Read more (PubMed: 20147388) »

See 1 Publication for this product

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