• Product name
    Anti-CD40 antibody [B-B20] (FITC)
    See all CD40 primary antibodies
  • Description
    Mouse monoclonal [B-B20] to CD40 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody recognises the Bp50 antigen
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Common marmoset
  • Immunogen

    Raji cell line (Human)

  • Positive control
    • Human lymphocytes



Our Abpromise guarantee covers the use of ab27281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. or 100 µl of whole blood.

ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Function
    Receptor for TNFSF5/CD40LG.
  • Tissue specificity
    B-cells and in primary carcinomas.
  • Involvement in disease
    Defects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similarities
    Contains 4 TNFR-Cys repeats.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody [B-B20] (FITC) images

  • A typical staining pattern with the B-B20 monoclonal antibody of lymphocytes

References for Anti-CD40 antibody [B-B20] (FITC) (ab27281)

This product has been referenced in:
  • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . Read more (PubMed: 25622566) »
  • Kap YS  et al. CD20+ B cell depletion alters T cell homing. J Immunol 192:4242-53 (2014). Flow Cyt ; Common marmoset . Read more (PubMed: 24696233) »

See all 3 Publications for this product

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Ok, thanks for letting me know. The Anti-CD40 PE antibody ab27282 should be stored at +4°C (not suitable for freezing) and we would expect this to remain active up to a year after purchase. The Anti-CD40 FITC antibody ab27281 can be aliquotted and stor...

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Thank you for contacting us. I'm sorry that you have been experiencing problems with Anti-CD40 antibody [B-B20] (Phycoerythrin) (ab27282). I have checked our records and we haven't had any reports of the antibody not working but as you have been able t...

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