• Product nameAnti-CD40 antibody [B-B20] (FITC)
    See all CD40 primary antibodies
  • Description
    Mouse monoclonal [B-B20] to CD40 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody recognises the Bp50 antigen
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Marmoset (common)
  • Immunogen

    Raji cell line (Human)

  • Positive control
    • Human lymphocytes



Our Abpromise guarantee covers the use of ab27281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. or 100 µl of whole blood.

ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor for TNFSF5/CD40LG.
  • Tissue specificityB-cells and in primary carcinomas.
  • Involvement in diseaseDefects in CD40 are the cause of hyper-IgM immunodeficiency syndrome type 3 (HIGM3) [MIM:606843]; also known as hyper-IgM syndrome 3. HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Sequence similaritiesContains 4 TNFR-Cys repeats.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI326936 antibody
    • B cell associated molecule CD40 antibody
    • B cell surface antigen CD40 antibody
    • B cell-associated molecule antibody
    • B-cell surface antigen CD40 antibody
    • Bp50 antibody
    • CD 40 antibody
    • CD40 antibody
    • CD40 antigen (TNF receptor superfamily member 5) antibody
    • CD40 antigen antibody
    • CD40 molecule antibody
    • CD40 molecule, TNF receptor superfamily member 5 antibody
    • CD40 protein antibody
    • CD40 type II isoform antibody
    • CD40L receptor antibody
    • CDw40 antibody
    • GP39 antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • MGC9013 antibody
    • Nerve growth factor receptor related B lymphocyte activation molecule antibody
    • OTTHUMP00000031699 antibody
    • OTTHUMP00000031700 antibody
    • p50 antibody
    • T-BAM antibody
    • TBAM antibody
    • TNF receptor superfamily member 5 antibody
    • TNFRSF5 antibody
    • TNR5_HUMAN antibody
    • TRAP antibody
    • Tumor necrosis factor receptor superfamily , member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 antibody
    • Tumor necrosis factor receptor superfamily member 5 precursor antibody
    • Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody
    see all

Anti-CD40 antibody [B-B20] (FITC) images

  • A typical staining pattern with the B-B20 monoclonal antibody of lymphocytes

References for Anti-CD40 antibody [B-B20] (FITC) (ab27281)

This product has been referenced in:
  • Toyoshima K  et al. Analysis of circulating tumor cells derived from advanced gastric cancer. Int J Cancer 137:991-8 (2015). Flow Cyt ; Human . Read more (PubMed: 25622566) »
  • Kap YS  et al. CD20+ B cell depletion alters T cell homing. J Immunol 192:4242-53 (2014). Flow Cyt ; Marmoset (common) . Read more (PubMed: 24696233) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

We will send you the concentration details soon.

Many thanks for having patience!

Ok, thanks for letting me know. The Anti-CD40 PE antibody ab27282 should be stored at +4°C (not suitable for freezing) and we would expect this to remain active up to a year after purchase. The Anti-CD40 FITC antibody ab27281 can be aliquotted and stor...

Read More

Thank you for contacting us. I'm sorry that you have been experiencing problems with Anti-CD40 antibody [B-B20] (Phycoerythrin) (ab27282). I have checked our records and we haven't had any reports of the antibody not working but as you have been able t...

Read More