Overview

  • Product name
  • Description
    Rabbit polyclonal to CD40L
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N terminal of Human CD40L, different from the relative mouse sequence by two amino acids.

  • Positive control
    • IHC-P: Mammary cancer sections Wb: Rat spleen tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab65854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificity
    Specifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in disease
    Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 40L antibody
    • CD154 antibody
    • CD40 antigen ligand antibody
    • CD40 ligand antibody
    • CD40 ligand, soluble form antibody
    • CD40-L antibody
    • CD40L antibody
    • CD40L_HUMAN antibody
    • CD40LG antibody
    • gp39 antibody
    • hCD40L antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • T B cell activating molecule antibody
    • T BAM antibody
    • T-cell antigen Gp39 antibody
    • TNF-related activation protein antibody
    • TNFSF5 antibody
    • TrAP antibody
    • Tumor necrosis factor (ligand) superfamily member 5 antibody
    • Tumor necrosis factor ligand superfamily member 5 antibody
    see all

Images

  • ab65854, at 2µg/ml, staining CD40L in the cytoplasm of paraffin embedded mammary cancer tissue by Immunohistochemistry. DAB chromogenic reaction.
  • Anti-CD40L antibody (ab65854) at 2 µg/ml + Rat spleen

    Predicted band size : 29 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa,38 kDa,39 kDa. We are unsure as to the identity of these extra bands.


  • Predicted band size : 29 kDa

References

This product has been referenced in:
  • Guiraut C  et al. Perinatal Arterial Ischemic Stroke Is Associated to Materno-Fetal Immune Activation and Intracranial Arteritis. Int J Mol Sci 17:N/A (2016). IHC-P ; Rat . Read more (PubMed: 27898024) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (MCF-7)
Specification
MCF-7
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Sample
Human Tissue sections (Breast carcinoma)
Specification
Breast carcinoma
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 16 2013

Thank you once again for your follow up email.

I am pleased to let you know I have now heard back from the originator with a copy of the general IHC-P protocol that they use for testing. Please note this will be a guideline only and may requ...

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Thank you for contacting us.
The antibody ab104686 is not in clear solution it is indeed in whole antiserum. We would recommend vortexing the antibody for a while and then after centrifuging using it as normal product. If in any case the antibody ...

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Thank you for taking the time to complete our questionnaire. I am sorry to hear you have had difficulty submitting and sending the information to us. I can confirm we have now received the questionnaire and the images from you. I appreciate your patien...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forIHC-...

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Thank you for contacting Abcam regarding our CD40 and CD40L antibodies. Below please find the requested information: ab2391 Rabbit Polyclonal to CD40L - Unfortunately the specific immunogen sequence is proprietary, however I can inform you that...

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Thank you for your enquiry. Please find the protocols as below: 1.Cover the entire surface of a clean microslide with APES or POLY-L-LYSINE. Incubate for 1 minute then rinse the microslide with water. Mount a tissue section (~5μm thick) with the tr...

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