Anti-CD40L antibody (Biotin) (ab83152)

Overview

  • Product nameAnti-CD40L antibody (Biotin)
    See all CD40L primary antibodies
  • Description
    Rabbit polyclonal to CD40L (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human CD40L

Properties

Applications

Our Abpromise guarantee covers the use of ab83152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
  • Application notesELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human CD40L.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human CD40L.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human CD40L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
      Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
    • Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
    • Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
    • Sequence similaritiesBelongs to the tumor necrosis factor family.
    • Post-translational
      modifications
      The soluble form derives from the membrane form by proteolytic processing.
      N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
      Not O-glycosylated.
    • Cellular localizationSecreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 40L antibody
      • CD154 antibody
      • CD40 antigen ligand antibody
      • CD40 ligand antibody
      • CD40 ligand, soluble form antibody
      • CD40-L antibody
      • CD40L antibody
      • CD40L_HUMAN antibody
      • CD40LG antibody
      • gp39 antibody
      • hCD40L antibody
      • HIGM1 antibody
      • IGM antibody
      • IMD3 antibody
      • T B cell activating molecule antibody
      • T BAM antibody
      • T-cell antigen Gp39 antibody
      • TNF-related activation protein antibody
      • TNFSF5 antibody
      • TrAP antibody
      • Tumor necrosis factor (ligand) superfamily member 5 antibody
      • Tumor necrosis factor ligand superfamily member 5 antibody
      see all

    References for Anti-CD40L antibody (Biotin) (ab83152)

    ab83152 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    First, I apologize but the immunogen I passed along was the sequence of the entire CD40 protein. However the actual immunogen sequence only consists of the soluble form of the protein, amino acids 113 - 261. I have i...

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