The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 27882144
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Is unsuitable for Flow Cyt or ICC.
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Specifically expressed on activated CD4+ T-lymphocytes.
Involvement in disease
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Belongs to the tumor necrosis factor family.
The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated.
Tumor necrosis factor (ligand) superfamily member 5 antibody
Tumor necrosis factor ligand superfamily member 5 antibody
Western blot - Anti-CD40L antibody [EP462E] (ab52750)
Anti-CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 cell lysate at 10 µg
Secondary Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 36 kDa Observed band size: 36 kDa
This product has been referenced in:
Sun KT et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep7:202 (2017).
Read more (PubMed: 28303002) »
Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med12:3242-3248 (2016).
Read more (PubMed: 27882144) »