The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsThe soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated.
Tumor necrosis factor (ligand) superfamily member 5 antibody
Tumor necrosis factor ligand superfamily member 5 antibody
Anti-CD40L antibody [EP462E] images
Western blot - CD40L antibody [EP462E] (ab52750)
Anti-CD40L antibody [EP462E] (ab52750) at 1/5000 dilution + MOLT-4 cell lysate at 10 µg
Secondary Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 36 kDa Observed band size : 36 kDa
References for Anti-CD40L antibody [EP462E] (ab52750)
This product has been referenced in:
Wang X et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med12:3242-3248 (2016).
Read more (PubMed: 27882144) »