Anti-CD40L antibody [MR1] (FITC) (ab24934)

Overview

  • Product name
    Anti-CD40L antibody [MR1] (FITC)
    See all CD40L primary antibodies
  • Description
    Armenian hamster monoclonal [MR1] to CD40L (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab24934 recognises CD40L.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: Activated mouse Th1 clone D1.6.

  • Positive control
    • Partially purified spleen T cells from BALB/c mice.

Properties

Applications

Our Abpromise guarantee covers the use of ab24934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificity
    Specifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in disease
    Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 40L antibody
    • CD154 antibody
    • CD40 antigen ligand antibody
    • CD40 ligand antibody
    • CD40 ligand, soluble form antibody
    • CD40-L antibody
    • CD40L antibody
    • CD40L_HUMAN antibody
    • CD40LG antibody
    • gp39 antibody
    • hCD40L antibody
    • HIGM1 antibody
    • IGM antibody
    • IMD3 antibody
    • T B cell activating molecule antibody
    • T BAM antibody
    • T-cell antigen Gp39 antibody
    • TNF-related activation protein antibody
    • TNFSF5 antibody
    • TrAP antibody
    • Tumor necrosis factor (ligand) superfamily member 5 antibody
    • Tumor necrosis factor ligand superfamily member 5 antibody
    see all

References

ab24934 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (lymphocytes and granulocytes)
Specification
lymphocytes and granulocytes
Permeabilization
No
Username

Mrs. Ann Kelly

Verified customer

Submitted Dec 05 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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