The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. In vivo and in vitro blocking of CD40L function.
Use 2µg for 106 cells. ab18473-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Sequence similaritiesBelongs to the tumor necrosis factor family.
Post-translational modificationsThe soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated.
Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or plate bound hamster anti mouse CD3 epsilon for 7 hours at 37°C. The cells were then harvested, double stained with ab24934 (2µg for 106 cells) and anti CD4+8 PE, and analyzed by flow cytometry.
References for Anti-CD40L antibody [MR1] (FITC) (ab24934)
has not yet been referenced specifically in any publications.
Publishing research using ab24934? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"