Overview

  • Product nameCD40L Human ELISA Kit
    See all CD40L kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.35 86% - 105%
    Serum 93.57 83% - 103%
    Plasma 94.63 84% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD40L Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40L in serum, plasma (EDTA or heparin), and cell culture supernatants.

    This assay employs an antibody specific for Human CD40L coated on a 96-well plate. Standards and samples are pipetted into the wells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
  • Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
  • Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
  • Sequence similaritiesBelongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • CD154
    • CD40 antigen ligand
    • CD40 ligand
    • CD40-L
    • CD40L_HUMAN
    • CD40LG
    • gp39
    • HIGM1
    • IGM
    • IMD3
    • soluble form
    • T B cell activating molecule
    • T BAM
    • T cell antigen Gp39
    • T-cell antigen Gp39
    • TNF related activation protein
    • TNF-related activation protein
    • TNFSF5
    • TrAP
    • Tumor necrosis factor (ligand) superfamily member 5
    • Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
    • Tumor necrosis factor ligand superfamily member 5
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD40L Human ELISA Kit images

  • Representative Standard Curve using ab99991.

  • Representative Standard Curve using ab99991.

Protocols

References for CD40L Human ELISA Kit (ab99991)

ab99991 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. This kit does recognize the extracellular region of CD40L (aa 47 – 261). Thus, it is used for the detection of soluble CD40L. The exact cross-reactivity of membrane-bound CD40L has not been tested. Please let me know if you...

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Merci de votre patience.
Le laboratoire m'avait envoyé les données qu'ils ont obtenu avec ce lot. Veuillez les trouves dans le document attaché. Les résultats sont mieux que ceux que vous avez obtenue avec le kit que nous vous avions envoyé. Si vo...

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Thank you for contacting us. We unfortunately can’t provide aspecific starting dilution factorat whichthere will be no interference from other plasma proteins in the assay.We recommend trying a series of dilutions until a linear response is measu...

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Thank you for your reply. As I mentioned in my previous email, we do not have a recommended minimum dilution for this kit because the amount of dilution necessary will depend on your samples.  Our recommended starting dilution for further optimization ...

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Thank you for contacting us.  Matrix effects can sometimes occur when certain factors in the plasma such as auto-antibodies, binding proteins, albumin, etc. can interfere with the assay if too much is present in your samples.  The result is that the sa...

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Merci de nous avoir contactés. J'ai reçu les informations suivantes concernant les kits ELISA CD40L et PF4 : ab99991 doit être utilisé avec des échantillons de plasma traités EDTA ou héparine. L'utilisation d'autres anticoagulants n'est pas recomma...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"