CD40L Mouse ELISA Kit (ab119517)
- Product nameCD40L Mouse ELISA KitSee all CD40L kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 8 6.5% Overall 8 6.5% Inter-assay Sample n Mean SD CV% Overall 8 11.1% Overall 8 11.1%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich (quantitative)
- Sensitivity0.14 ng/ml
- Range0.31 ng/ml - 20 ng/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s CD40L Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Mouse CD40L concentrations in cell culture supernatant and serum.
CD40L specific antibodies have been precoated onto 96-well plates. Standards or test samples are added to the wells and subsequently a CD40L specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of CD40L captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-mouse CD40L monoclonal antibody 1 x 100µl Blue-Dye 1 x 400µl Green-Dye 1 x 400µl Microplate coated with monoclonal antibody to mouse CD40L (12 x 8 wells) 1 unit Mouse CD40L Standard lyophilized (40 ng /mL upon reconstitution) 2 vials Red-Dye 1 x 400µl Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml
- Research Areas
- FunctionMediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
- Tissue specificitySpecifically expressed on activated CD4+ T-lymphocytes.
- Involvement in diseaseDefects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
- Sequence similaritiesBelongs to the tumor necrosis factor family.
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
- Cellular localizationSecreted and Cell membrane.
- CD40 antigen ligand
- CD40 ligand
- soluble form
- T B cell activating molecule
- T BAM
- T cell antigen Gp39
- T-cell antigen Gp39
- TNF related activation protein
- TNF-related activation protein
- Tumor necrosis factor (ligand) superfamily member 5
- Tumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome)
- Tumor necrosis factor ligand superfamily member 5
Our Abpromise guarantee covers the use of ab119517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD40L Mouse ELISA Kit (ab119517)
ab119517 has not yet been referenced specifically in any publications.