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The details of the immunogen for this antibody are not available.
Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo.
Our Abpromise guarantee covers the use of ab33661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 20525356|
|Flow Cyt||1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
ab33661 staining CD41 in mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA in PBS for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 9 hours at 4°C. A Biotin-conjugated goat anti-rat IgG polyclonal (1/300) was used as the secondary antibody.
IHC-Fr image of CD41 staining on mouse lung sections using ab33661 (1:300). The the sections came from PFA fixed and snap frzen tissue and 10 micron sections were cut using a cryostat. The sections were blocked with 1% BSA at 21°C for 10 mins. The sections were then incubated with ab33661 for 2 hours at 21°C. Goat Polyclonal to Rat IgG conjugated to Biotin was used at 1:200.