PE Anti-CD41 antibody [MWReg30] (ab33660)
Key features and details
- PE Rat monoclonal [MWReg30] to CD41
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PE Anti-CD41 antibody [MWReg30]
See all CD41 primary antibodies -
Description
PE Rat monoclonal [MWReg30] to CD41 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab33660 recognizes integrin alpha IIb subunit CD41. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo. The azide free format is recommended for functional studies.
Purified IgG conjugated to R. Phycoerythrin (RPE).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant -
Primary antibody notes
Clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo. The azide free format is recommended for functional studies. -
Clonality
Monoclonal -
Clone number
MWReg30 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Use 10μl of the suggested working dilution to label 106 cells in 100μl. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Use 10μl of the suggested working dilution to label 106 cells in 100μl. |
Target
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Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. -
Tissue specificity
Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. -
Involvement in disease
Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16399 Mouse
- SwissProt: Q9QUM0 Mouse
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Alternative names
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab33660 has been referenced in 2 publications.
- Mobarrez F et al. Microparticles in the blood of patients with systemic lupus erythematosus (SLE): phenotypic characterization and clinical associations. Sci Rep 6:36025 (2016). PubMed: 27777414
- Nieswandt B et al. Acute systemic reaction and lung alterations induced by an antiplatelet integrin gpIIb/IIIa antibody in mice. Blood 94:684-93 (1999). PubMed: 10397735