Overview

  • Product name
  • Description
    Rabbit polyclonal to CD42b
  • Tested applications
    Suitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 573-622 (RGSLPTFRSS LFLWVRPNGR VGPLVAGRRP SALSQGRGQD LLSTVSIRYS) of Human CD42b (NP_000164)

  • Positive control
    • Jurkat cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Antigen CD42b alpha antibody
    • BDPLT1 antibody
    • BDPLT3 antibody
    • BSS antibody
    • CD 42b antibody
    • CD42b alpha antibody
    • CD42B antibody
    • CD42b antigen antibody
    • DBPLT3 antibody
    • GLYCOCALICIN antibody
    • Glycoprotein Ib (platelet) alpha polypeptide antibody
    • Glycoprotein Ibalpha antibody
    • GP Ib alpha antibody
    • GP1B antibody
    • GP1BA antibody
    • GPIb alpha antibody
    • GPIbA antibody
    • MGC34595 antibody
    • Platelet glycoprotein Ib alpha chain antibody
    • Platelet glycoprotein Ib alpha polypeptide antibody
    • Platelet membrane glycoprotein 1b alpha subunit antibody
    • VWDP antibody
    see all

Images

  • IHC-Fr image of CD42b staining on human blood film using ab104704 (1:1000). The blood was fixed uisng paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. Blocking was performed using 10 % Donkey serum for 1 hour at 24°C. The primary antibody was diluted 1:1000 using 0.1% TritonX with 0.1x PBS and 10% Donkey serum; incubated for 4 hours at 24°C. The Secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)

    See Abreview

  • Anti-CD42b antibody (ab104704) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 69 kDa
    Gel concentration: 12%

References

ab104704 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization
No
Specification
liver cancer
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 19 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Sample
Mouse Tissue sections (platelets in fetal lung (E18.5))
Specification
platelets in fetal lung (E18.5)
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 03 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Human blood film)
Specification
Human blood film
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
20 µg
Specification
platelet
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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