The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Use neat - 1/10. Use 10µl of the suggested working dilution to label 100µl whole blood.
ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceCD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
FACS analysis of CD42b expressing cells in human peripheral blood platelets using ab30401.
References for Anti-CD42b antibody [AK2] (FITC) (ab30401)
This product has been referenced in:
Kumar RA et al. Kinetics of GPIbalpha-vWF-A1 tether bond under flow: effect of GPIbalpha mutations on the association and dissociation rates. Biophys J85:4099-109 (2003).
Read more (PubMed: 14645097) »