Anti-CD42b antibody [MM2/174] (FITC) (ab82594)

Overview

  • Product nameAnti-CD42b antibody [MM2/174] (FITC)
    See all CD42b primary antibodies
  • Description
    Mouse monoclonal [MM2/174] to CD42b (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: WB, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab82594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceCD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • Antigen CD42b alpha antibody
    • BDPLT1 antibody
    • BDPLT3 antibody
    • BSS antibody
    • CD 42b antibody
    • CD42b alpha antibody
    • CD42B antibody
    • CD42b antigen antibody
    • DBPLT3 antibody
    • GLYCOCALICIN antibody
    • Glycoprotein Ib (platelet) alpha polypeptide antibody
    • Glycoprotein Ibalpha antibody
    • GP Ib alpha antibody
    • GP1B antibody
    • GP1BA antibody
    • GPIb alpha antibody
    • GPIbA antibody
    • MGC34595 antibody
    • Platelet glycoprotein Ib alpha chain antibody
    • Platelet glycoprotein Ib alpha polypeptide antibody
    • Platelet membrane glycoprotein 1b alpha subunit antibody
    • VWDP antibody
    see all

Anti-CD42b antibody [MM2/174] (FITC) images

  • Anti-CD42b antibody [MM2/174] (FITC) (ab82594) at 1/300 dilution + Human Platelets

    Secondary
    Goat Polyclonal to mouse IgG (IRDye 680) - undiluted

    Performed under reducing conditions.

    Predicted band size : 69 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous abreview.

    Detection method : Licor

    See Abreview

References for Anti-CD42b antibody [MM2/174] (FITC) (ab82594)

ab82594 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your reply.

At this time we don't offer custom conjugation on single antibodies, only on bulk orders (greater than 10 vials), so we only have the unconjugated version of ab104704. To use this antibody, a PE conjugated ...

Read More

Thank you very much for your call earlier this week and for your patience while I have been in touch with the lab regarding your enquiry.

The human CD42b sequence is only 61% identical to the porcine sequence, so we would only predict that an...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Loading amount 20 µg
Specification platelet
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Submitted Aug 13 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"