Overview

  • Product name
    Anti-CD42b antibody [VM16d]
    See all CD42b primary antibodies
  • Description
    Mouse monoclonal [VM16d] to CD42b
  • Tested applications
    Suitable for: ICC/IF, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation containing human CD42b glycoprotein.

  • Epitope
    The epitope is located within the 45 kDa N-terminal domain of GPIb alpha- chain.
  • General notes


    This antibody inhibits low conc. thrombin (<0.1 U/ml) induced platelet aggregation and decreases thrombin binding to platelets. F(ab')2 and F(ab') fragments are equally effective as whole intact antibodies. It does not affect platelet interaction with von Willebrand factor, leading to the conclusion that the antibody reacts with or nearby the thrombin receptor site.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes
    Ammonium sulfate + DEAE-cellulose, gradient elution
  • Primary antibody notes
    This antibody inhibits low conc. thrombin (<0.1 U/ml) induced platelet aggregation and decreases thrombin binding to platelets. F(ab')2 and F(ab') fragments are equally effective as whole intact antibodies. It does not affect platelet interaction with von Willebrand factor, leading to the conclusion that the antibody reacts with or nearby the thrombin receptor site.
  • Clonality
    Monoclonal
  • Clone number
    VM16d
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

Target

  • Relevance
    CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. Its expression and activation seems to be regulated by VEGF and PDGF.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Antigen CD42b alpha antibody
    • BDPLT1 antibody
    • BDPLT3 antibody
    • BSS antibody
    • CD 42b antibody
    • CD42b alpha antibody
    • CD42B antibody
    • CD42b antigen antibody
    • DBPLT3 antibody
    • GLYCOCALICIN antibody
    • Glycoprotein Ib (platelet) alpha polypeptide antibody
    • Glycoprotein Ibalpha antibody
    • GP Ib alpha antibody
    • GP1B antibody
    • GP1BA antibody
    • GPIb alpha antibody
    • GPIbA antibody
    • MGC34595 antibody
    • Platelet glycoprotein Ib alpha chain antibody
    • Platelet glycoprotein Ib alpha polypeptide antibody
    • Platelet membrane glycoprotein 1b alpha subunit antibody
    • VWDP antibody
    see all

Anti-CD42b antibody [VM16d] images

  • Anti-CD42b antibody [VM16d] (ab34408) at 1/50 dilution + Human platelet lysate

    Secondary
    Odyssey goat polyclonal to mouse IgG (IRDye 680)

    Performed under reducing conditions.

    Exposure time : 3 minutes

    This image is courtesy of an anonymous abreview.

    Detection method: Licor System

    See Abreview

References for Anti-CD42b antibody [VM16d] (ab34408)

This product has been referenced in:
  • Mazurov AV  et al. Characterization of an antiglycoprotein Ib monoclonal antibody that specifically inhibits platelet-thrombin interaction. Thromb Res 62:673-84 (1991). Read more (PubMed: 1718052) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
20 µg
Specification
platelet
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2012

Thank you for your recent telephone enquiry.  I have obtained the following information: The following CD42b antibodies have been tested and guaranteed in functional studies: ab2578 CD42b antibody [HIP1] Click here (or use the f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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