Overview

  • Product nameAnti-CD42d antibody
    See all CD42d primary antibodies
  • Description
    Rabbit polyclonal to CD42d
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human CD42d.

    (Peptide available as ab74425.)

  • Positive control
    • This antibody gave a positive signal in the following Lysates: EBS E14 TG2A Day 8 Whole Cell, Raji Whole Cell, EBS E14 TG2A Day 0 Whole Cell, EBS E14 TG2A Day 5 Whole Cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab65017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 61 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionThe GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis.
  • Tissue specificityPlatelets and megakaryocytes.
  • Sequence similaritiesContains 13 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 42d antibody
    • CD42d antibody
    • CD42d antigen antibody
    • Glycoprotein 5 antibody
    • Glycoprotein V antibody
    • Glycoprotein V platelet antibody
    • GlycoproteinV antibody
    • Gp 5 antibody
    • GP V antibody
    • GP5 antibody
    • GPV antibody
    • GPV_HUMAN antibody
    • Platelet glycoprotein V antibody
    • Platelet glycoprotein V precursor antibody
    see all

Anti-CD42d antibody images

  • All lanes : Anti-CD42d antibody (ab65017) at 1 µg/ml

    Lane 1 : EBS E14 TG2A Day 8 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : EBS E14 TG2A Day 0 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate
    Lane 4 : EBS E14 TG2A Day 5 (Mouse Pluripotent Embryonic Stem Cell) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-CD42d antibody (ab65017)

ab65017 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization Yes - 0.1% Triton X-100
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative Paraformaldehyde
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Submitted Dec 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"