Overview

  • Product nameAnti-CD43 antibody [DF-T1]
    See all CD43 primary antibodies
  • Description
    Mouse monoclonal [DF-T1] to CD43
  • SpecificityWe have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human) - Myeloblastic KG1 cells

  • Positive control
    • Human tonsil or lymph node.
  • General notes


    ab76524 can be useful in identification and classification of T-cell malignancies.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 4 µg/ml.

For 30 min at RT. No special pretreatment is required for immunohistochemical staining of formalin-fixed tissues.

Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications
    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

References for Anti-CD43 antibody [DF-T1] (ab76524)

This product has been referenced in:
  • Stross WP  et al. Molecule detected in formalin fixed tissue by antibodies MT1, DF-T1, and L60 (Leu-22) corresponds to CD43 antigen. J Clin Pathol 42:953-61 (1989). Read more (PubMed: 2794085) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"