Overview

  • Product nameAnti-CD43 antibody [MEM-59]
    See all CD43 primary antibodies
  • Description
    Mouse monoclonal [MEM-59] to CD43
  • SpecificityRecognizes neuraminidase-sensitive epitope on human CD43 molecule (Leukosialin), which is involved in lymphocyte activation.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IP, WB, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD43.

  • Positive control
    • Positive tissue: spleen, thymus, tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab9088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
WB 1/100. Use under non-reducing conditions.
Functional Studies Use at an assay dependent concentration. The antibody activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells.

Target

  • FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications
    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

References for Anti-CD43 antibody [MEM-59] (ab9088)

This product has been referenced in:
  • Cermak L  et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem 277:7955-61 (2002). Read more (PubMed: 11773067) »
  • Alvarado M  et al. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol 25:1051-5 (1995). Read more (PubMed: 7537669) »

See all 5 Publications for this product

Product Wall

Thank you for your email. Human T lymphocytes were used as the immunogen to generate ab9088. This antibody recognizes neuraminidase-sensitive epitope on human CD43 molecule (Leukosialin), which is involved in lymphocyte activation. If you have any a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"