Overview

  • Product nameAnti-CD43 antibody [MEM-59] (Biotin)
    See all CD43 primary antibodies
  • Description
    Mouse monoclonal [MEM-59] to CD43 (Biotin)
  • ConjugationBiotin
  • SpecificityThe antibody recognizes neuraminidase sensitive epitope on CD43 antigen (Leukosialin), which is involved in lymphocyte activation.
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation: T lymphocytes (Human).

  • General notes

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Properties

Applications

Our Abpromise guarantee covers the use of ab28104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/200. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications
    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

References for Anti-CD43 antibody [MEM-59] (Biotin) (ab28104)

This product has been referenced in:
  • Alvarado M  et al. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol 25:1051-5 (1995). Read more (PubMed: 7537669) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"