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Overview

  • Product nameAnti-CD43 antibody [MEM-59] (FITC)
    See all CD43 primary antibodies
  • Description
    Mouse monoclonal [MEM-59] to CD43 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Tissue/ cell preparation (T lymphocytes) (Human).

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Properties

Applications

Our Abpromise guarantee covers the use of ab21853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD 43 antibody
      • CD43 antibody
      • CD43 antigen antibody
      • Galactoglycoprotein antibody
      • GALGP antibody
      • GPL 115 antibody
      • GPL115 antibody
      • Human gene for sialophorin antibody
      • Leucocyte sialoglycoprotein antibody
      • LEUK_HUMAN antibody
      • Leukocyte large sialoglycoprotein antibody
      • Leukocyte sialoglycoprotein antibody
      • Leukosialin antibody
      • LSN antibody
      • Ly-48 antibody
      • sialophorin (gpL115, leukosialin, CD43) antibody
      • Sialophorin antibody
      • Spn antibody
      see all

    Anti-CD43 antibody [MEM-59] (FITC) images

    • Flow Cytometry - Anti-CD43 antibody [MEM-59] (FITC) (ab21853)
      Flow Cytometry - Anti-CD43 antibody [MEM-59] (FITC) (ab21853)
      Flow Cytometry analysis of human peripheral blood cells labeling CD43 with Anti-CD43 antibody [MEM-59] (FITC) (ab21853).
    • Flow Cytometry-Anti-CD43 antibody [MEM-59] (FITC)(ab21853)
      Flow Cytometry-Anti-CD43 antibody [MEM-59] (FITC)(ab21853)
      Overlay histogram showing peripheral blood lymphocytes stained with ab21853(red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21853, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

    References for Anti-CD43 antibody [MEM-59] (FITC) (ab21853)

    This product has been referenced in:
    • Tingling JD  et al. CD24 expression identifies teratogen-sensitive fetal neural stem cell subpopulations: evidence from developmental ethanol exposure and orthotopic cell transfer models. PLoS One 8:e69560 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23894503) »
    • Stracker TH  et al. The carboxy terminus of NBS1 is required for induction of apoptosis by the MRE11 complex. Nature 447:218-21 (2007). WB ; Mouse . Read more (PubMed: 17429352) »

    See all 2 Publications for this product

    Publishing research using ab21853? Please let us know so that we can cite the reference in this datasheet.

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    1 Abreview or Q&A

    10
    Votes: Highest First

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD43 antibody [MEM-59] (FITC)

     Inconclusive
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (embryonic stem cells)
    Specification embryonic stem cells
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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    Verified customer

    Submitted May 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"