Overview

  • Product nameAnti-CD43 antibody [MT1]
    See all CD43 primary antibodies
  • Description
    Mouse monoclonal [MT1] to CD43
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human lymphocytes.

  • Positive control
    • Human mantle cell lymphoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab89691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
  • Application notesIHC-P: 1/20 - 1/40.
    Trypsin digestion of paraffin sections may enhance staining in some cases. 60 minutes primary antibody incubation at 25oC. Standard ABC technique.
    IHC-Fr: 1/20 - 1/40.
    WB: 1/250 - 1/400. Predicted molecular weight: 40 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
    • Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
    • Post-translational
      modifications
      Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 43 antibody
      • CD43 antibody
      • CD43 antigen antibody
      • Galactoglycoprotein antibody
      • GALGP antibody
      • GPL 115 antibody
      • GPL115 antibody
      • Human gene for sialophorin antibody
      • Leucocyte sialoglycoprotein antibody
      • LEUK_HUMAN antibody
      • Leukocyte large sialoglycoprotein antibody
      • Leukocyte sialoglycoprotein antibody
      • Leukosialin antibody
      • LSN antibody
      • Ly-48 antibody
      • sialophorin (gpL115, leukosialin, CD43) antibody
      • Sialophorin antibody
      • Spn antibody
      see all

    Anti-CD43 antibody [MT1] images

    • ab89691 at 1/20 dilution staining CD43 in Human mantle cell lymphoma by Immunohistochemistry, Paraffin-embedded tissue. Note intense membrane staining of tumor cells

    References for Anti-CD43 antibody [MT1] (ab89691)

    This product has been referenced in:
    • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . Read more (PubMed: 26732624) »
    • Xiao LI  et al. Multiple myeloma-associated skin light chain amyloidosis: A case of misdiagnosis. Oncol Lett 11:3617-3620 (2016). IHC ; Human . Read more (PubMed: 27284363) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"