Anti-CD43 antibody [SP55], prediluted (ab101534)

Overview

  • Product nameAnti-CD43 antibody [SP55], prediluted
    See all CD43 primary antibodies
  • Description
    Rabbit monoclonal [SP55] to CD43, prediluted
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide derived from an internal region of Human CD43.

  • Positive control
    • Human Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab101534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Antigen retrieval is recommended: boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB Use at an assay dependent concentration.

Target

  • FunctionOne of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
  • Tissue specificityCell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
  • Post-translational
    modifications
    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 43 antibody
    • CD43 antibody
    • CD43 antigen antibody
    • Galactoglycoprotein antibody
    • GALGP antibody
    • GPL 115 antibody
    • GPL115 antibody
    • Human gene for sialophorin antibody
    • Leucocyte sialoglycoprotein antibody
    • LEUK_HUMAN antibody
    • Leukocyte large sialoglycoprotein antibody
    • Leukocyte sialoglycoprotein antibody
    • Leukosialin antibody
    • LSN antibody
    • Ly-48 antibody
    • sialophorin (gpL115, leukosialin, CD43) antibody
    • Sialophorin antibody
    • Spn antibody
    see all

Anti-CD43 antibody [SP55], prediluted images

  • Staining of Human CD43 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab101534 at a dilution of 1/1.
  • Anti-CD43 antibody [SP55] (ab101533) at 1/25 dilution + Jurkat cell lysate

    Observed band size : 105,160 kDa (why is the actual band size different from the predicted?)

References for Anti-CD43 antibody [SP55], prediluted (ab101534)

ab101534 has not yet been referenced specifically in any publications.

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