The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Antigen retrieval is recommended: boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Use at an assay dependent concentration.
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.