- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-CD44 antibody [156-3C11]
See all CD44 primary antibodies -
DescriptionMouse monoclonal [156-3C11] to CD44
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Human, Baboon
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Immunogen
Stimulated human leukocytes.
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Positive control
- Tonsil, HeLa cell line
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 1% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number156-3C11
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IsotypeIgG2a
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab16728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | 1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/1000. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
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Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
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Sequence similaritiesContains 1 Link domain.
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DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
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Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 101008690 Baboon
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
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![Flow Cytometry - Anti-CD44 antibody [156-3C11] (ab16728)](http://a.abcam.com/ps/products/16/ab16728/Images/ab16728-199442-ab16728v2FC.jpg)
Flow Cytometry - Anti-CD44 antibody [156-3C11] (ab16728)Human peripheral blood lymphocytes stained with ab16728 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab16728, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)](http://a.abcam.com/ps/products/16/ab16728/Images/ab167281.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)This image is courtesy of an anonymous Abreviewab16728 at 1/50 dilution staining baboon skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were fixed with formaldehyde and blocked with a 1% Hydrogen peroxide / Methanol solution prior to incubation with the antibody for 1 hour. A biotinylated swine anti-goat, mouse, rabbit antibody was used as the secondary. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)](http://a.abcam.com/ps/products/16/ab16728/Images/ab16728_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [156-3C11] (ab16728)ab16728 staining human tonsil by IHC-P. -
![Immunohistochemistry (Frozen sections) - Anti-CD44 antibody [156-3C11] (ab16728)](http://a.abcam.com/ps/products/16/ab16728/Images/CD44-Primary-antibodies-ab16728-8.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD44 antibody [156-3C11] (ab16728)Image courtesy of an anonymous Abreview.ab16728 staining CD44 in human glioma xenograft in mouse brain by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, permeabilized with 1% Triton X-100 and then blocked using 2% serum for 1 hour at 21°C. Samples were then incubated with ab16728 at a 1/50 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 647 conjugated goat-anti mouse polyclonal used at a 1/1000 dilution.
The image shows ab16728 in green, human nestin in red and DAPI in blue. -
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [156-3C11] (ab16728)](http://a.abcam.com/ps/products/16/ab16728/Images/CD44-Primary-antibodies-ab16728-9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [156-3C11] (ab16728)ICC/IF image of ab16728 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16728, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Fatima H et al. Parietal epithelial cell activation marker in early recurrence of FSGS in the transplant. Clin J Am Soc Nephrol 7:1852-8 (2012). IHC ; Human . Read more (PubMed: 22917699) »
- Smeets B et al. Parietal epithelial cells participate in the formation of sclerotic lesions in focal segmental glomerulosclerosis. J Am Soc Nephrol 22:1262-74 (2011). IHC ; Human . Read more (PubMed: 21719782) »
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