Overview

  • Product nameAnti-CD44 antibody [5035-41.1D] (FITC)
    See all CD44 primary antibodies
  • Description
    Mouse monoclonal [5035-41.1D] to CD44 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab23557 recognises CD44 found on >90% bone marrow cells and spleen cells, 56% of thymus cells and 90% of lymph node cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: Spleen cells from B6PL-Ly-2a/ Ly-3a B6-Ly-1a mice

  • General notes

    Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC).

Properties

Applications

Our Abpromise guarantee covers the use of ab23557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 10µl for 106 cells, or 100µl whole blood.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • Sequence similaritiesContains 1 Link domain.
    • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
    • Post-translational
      modifications
      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      see all

    Anti-CD44 antibody [5035-41.1D] (FITC) images

    • ab23557 staining CD44 in mouse bone marrow cells by Flow Cytometry. 10 uL primary antibody was added to 50 uL freshly isolated mouse bone marrow cell (C57bl6 background) suspension in PBS with 1% normal mouse serum (~500,000 cells in total). Gating strategy: mesenchymal stem cells.

      See Abreview

    References for Anti-CD44 antibody [5035-41.1D] (FITC) (ab23557)

    This product has been referenced in:
    • Bertho JM  et al. Influence on the mouse immune system of chronic ingestion of (137)Cs. J Radiol Prot 31:25-39 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21346294) »
    • Katebi M  et al. Adenosine A2A receptors play an active role in mouse bone marrow-derived mesenchymal stem cell development. J Leukoc Biol 85:438-44 (2009). Flow Cyt ; Mouse . Read more (PubMed: 19056861) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (mouse bone marrow cells)
    Specification mouse bone marrow cells
    Fixation non
    Permeabilization No
    Gating Strategy mesenchymal stem cells
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"