The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 10µl for 106 cells, or 100µl whole blood.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
hematopoietic cell E- and L-selectin ligand antibody
Heparan sulfate proteoglycan antibody
Hermes antigen antibody
homing function and Indian blood group system antibody
Hyaluronate receptor antibody
INLU-related p80 Glycoprotein antibody
Phagocytic glycoprotein 1 antibody
Phagocytic glycoprotein I antibody
Soluble CD44 antibody
Anti-CD44 antibody [5035-41.1D] (FITC) images
Flow Cytometry - CD44 antibody [5035-41.1D] (FITC) (ab23557)This image is courtesy of an anonymous Abreview
ab23557 staining CD44 in mouse bone marrow cells by Flow Cytometry. 10 uL primary antibody was added to 50 uL freshly isolated mouse bone marrow cell (C57bl6 background) suspension in PBS with 1% normal mouse serum (~500,000 cells in total). Gating strategy: mesenchymal stem cells.
References for Anti-CD44 antibody [5035-41.1D] (FITC) (ab23557)
This product has been referenced in:
Bertho JM et al. Influence on the mouse immune system of chronic ingestion of (137)Cs. J Radiol Prot31:25-39 (2011).
Read more (PubMed: 21346294) »
Katebi M et al. Adenosine A2A receptors play an active role in mouse bone marrow-derived mesenchymal stem cell development. J Leukoc Biol85:438-44 (2009).
Read more (PubMed: 19056861) »