Overview

  • Product nameAnti-CD44 antibody
    See all CD44 primary antibodies
  • Description
    Rabbit polyclonal to CD44
  • Tested applicationsSuitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CD44.
    (Peptide available as ab52122)

  • Positive control
    • HeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab24504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IP
  • Application notesIHC-Fr: Use at a concentration of 20 µg/ml.
    IP: Use at a concentration of 20 µg/ml.
    WB: Use at a concentration of 1-4 µg/ml. Predicted molecular weight: 82 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • Sequence similaritiesContains 1 Link domain.
    • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
    • Post-translational
      modifications
      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • LHR antibody
      • BA-1 antibody
      • CD 44 antibody
      • CD44 antibody
      • CD44 antigen antibody
      • CD44 molecule (Indian blood group) antibody
      • CD44 molecule antibody
      • CD44_HUMAN antibody
      • CDw44 antibody
      • Cell surface glycoprotein CD44 antibody
      • chondroitin sulfate proteoglycan 8 antibody
      • CSPG8 antibody
      • ECMR-III antibody
      • Epican antibody
      • Extracellular matrix receptor III antibody
      • GP90 lymphocyte homing/adhesion receptor antibody
      • HCELL antibody
      • hematopoietic cell E- and L-selectin ligand antibody
      • Heparan sulfate proteoglycan antibody
      • Hermes antigen antibody
      • homing function and Indian blood group system antibody
      • HSA antibody
      • HUTCH-I antibody
      • HUTCH1 antibody
      • Hyaluronate receptor antibody
      • IN antibody
      • MC56 antibody
      • MDU2 antibody
      • MDU3 antibody
      • MGC10468 antibody
      • MIC4 antibody
      • MUTCH1 antibody
      • PGP-1 antibody
      • PGP-I antibody
      • PGP1 antibody
      • Phagocytic glycoprotein 1 antibody
      • Phagocytic glycoprotein I antibody
      see all

    Anti-CD44 antibody images

    • All lanes : Anti-CD44 antibody (ab24504) at 2 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 82 kDa
      Observed band size : 82 kDa
      Additional bands at : 25-50 kDa (possible isoform).
    • Immunohistochemical analysis of Human prostate cancer cell xenograft tissue, staining CD44 (red) with ab24504.

      Tissue was fixed with paraformaldehyde, permeabilized with 0.4% Triton X-100 and blocked with 10% serum. Samples were incubated overnight with primary antibody (1/100) overnight at 4°C. An AlexaFluor®555-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.

    References for Anti-CD44 antibody (ab24504)

    This product has been referenced in:
    • Kennedy E  et al. Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells? Vasc Cell 6:6 (2014). Rat . Read more (PubMed: 24628920) »
    • Zakharchenko O  et al. A role of TGFß1 dependent 14-3-3s phosphorylation at Ser69 and Ser74 in the regulation of gene transcription, stemness and radioresistance. PLoS One 8:e65163 (2013). IHC-Fr ; Human . Read more (PubMed: 23741479) »

    See all 8 Publications for this product

    Product Wall

    Thank you for contacting us.

    The link actually does not show results, I am sorry for the inconvenience.

    Alternatively you can type CD44 in the search box provided on the website. The system will automatically give you sorted anti C...

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    Thank you for your enquiry. At this point we do not have any data from testing the different isoform specificity of this antibody. All of our products are guaranteed to work in species and application as stated on the datasheet. Should you not ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (lung)
    Specification lung
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Mr. Pete Geisen

    Verified customer

    Submitted Jan 07 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (retina)
    Specification retina
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Mr. Pete Geisen

    Verified customer

    Submitted Jan 07 2008

    Thank you for your enquiry. HeLa cell extract can be used as a positive control with this antibody. Please contact us again if you have any additional questions.

    Thank you for getting back to me with an image of your blot. Once again I am sorry to hear that you have been having difficulties with this antibody. Your image is very convincing and the fact that you are not observing a band of 82KDa from a murin...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody by IP. I noticed that you have also made an enquiry about this antibody by western blotting. Quality is important to Abcam. I would appreciate ...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your questionnaire and I have a few comments. It would greatly help me if you could provide me with a quantitation value of th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"