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The details of the immunogen for this antibody are not available.
ab25224 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.
Our Abpromise guarantee covers the use of ab25224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab154449-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 80 - 95 kDa.|
Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25224 (Phycoerythrin) at 0.1 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG1-Phycoerythrin.