Overview

  • Product nameAnti-CD44 antibody [KM81]
    See all CD44 primary antibodies
  • Description
    Rat monoclonal [KM81] to CD44
  • Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, Inhibition Assay, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse Bone Marrow Derived Stromal Cells (clone BMS2).

  • Positive control
    • Mouse Bone Marrow cells (BALB/c strain). IHC-P: Mouse lung FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab112178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.2µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.

Target

  • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similaritiesContains 1 Link domain.
  • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [KM81] images

  • IHC image of CD44 staining in mouse lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112178, 1µg/ml, for 15 mins at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab112178, at 0.2 µg/106 cells, staining CD44 in Mouse Bone Marrow cells (strain: BALB/c) by Flow cytometry.
    Percentage of cells stained above control: 98.1%.
  • Immunofluorescence image of CD44 (ab112178) stained mouse mutant embryonic stem cells. The cells were paraformaldehyde fixed, before blocking in 0.1% BSA + 1% serum for 30 minutes at room temperature. Cells were then incubated with this antibody (ab112178, 1/100 dilution) for 2 hours at room temperature.

    See Abreview

References for Anti-CD44 antibody [KM81] (ab112178)

This product has been referenced in:
  • Hsieh IS  et al. MicroRNA-320 suppresses the stem cell-like characteristics of prostate cancer cells by downregulating the Wnt/beta-catenin signaling pathway. Carcinogenesis : (2012). Human . Read more (PubMed: 23188675) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cell (primary murine astrocytes)
Permeabilization Yes - 0.1% TritonX
Specification primary murine astrocytes
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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Verified customer

Submitted Dec 03 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako low pH buffer
Permeabilization No
Specification Mouse Lung
Blocking step Dako flex peroxidase block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Nov 24 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (HUCPVCs)
Specification HUCPVCs
Preparation Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy Live cells
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Verified customer

Submitted Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mutant embryonic stem cell)
Specification mutant embryonic stem cell
Fixative Paraformaldehyde
Permeabilization No
Blocking step 0.1% BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (mutant embryonic stem cell line expressing CD44)
Specification mutant embryonic stem cell line expressing CD44
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: cell dissociation buffer
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy live cells
Username

Abcam user community

Verified customer

Submitted Aug 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"