Anti-CD44 antibody [MEM-263] (Phycoerythrin) (ab58754)

Overview

  • Product name
    Anti-CD44 antibody [MEM-263] (Phycoerythrin)
    See all CD44 primary antibodies
  • Description
    Mouse monoclonal [MEM-263] to CD44 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab58754 reacts with the extracellular (N terminal) domain of standard CD44 present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Dog, Human, Pig, African green monkey
  • Immunogen

    Tissue/ cell preparation: Cos 7 cells (African Green Monkey).

  • Positive control
    • Peripheral blood lymphocytes from human, dog or pig. Kg-1a human acute leukemia cell lysate. JURKAT human leukemia T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use 20µl for 106 cells. (Good for 10^6 cells in suspension or for 100 µl of whole blood.)



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificity
    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similarities
    Contains 1 Link domain.
  • Domain
    The lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • INLU-related p80 Glycoprotein antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    • Soluble CD44 antibody
    see all

Images

  • All lanes : Anti-CD44 antibody [MEM-263] (Phycoerythrin) (ab58754) at 2 µg/ml

    Lane 1 : Human peripheral blood lymphocytes
    Lane 2 : Canine peripheral blood lymphocytes
    Lane 3 : Porcine peripheral blood lymphocytes

    Secondary
    anti-mouse IgG/HRP

    Predicted band size : 82 kDa
    Observed band size : 82 kDa

References

This product has been referenced in:
  • Linard C  et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med 2:916-27 (2013). Pig . Read more (PubMed: 24068742) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us. We do have products that are conjugated to match your needs, Ab81424 Anti-CD44 antibody [MEM-263] is conjugated with APC. Ab58754 Anti-CD44 antibody [MEM-263] (Phycoerythrin). I've included links to each below: http://www.a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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