Anti-CD44 antibody [MEM-263] (Phycoerythrin) (ab58754)


  • Product nameAnti-CD44 antibody [MEM-263] (Phycoerythrin)
    See all CD44 primary antibodies
  • Description
    Mouse monoclonal [MEM-263] to CD44 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab58754 reacts with the extracellular (N terminal) domain of standard CD44 present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Dog, Human, Pig, African Green Monkey
  • Immunogen

    Tissue/ cell preparation: Cos 7 cells (African Green Monkey).

  • Positive control
    • Peripheral blood lymphocytes from human, dog or pig. Kg-1a human acute leukemia cell lysate. JURKAT human leukemia T cell lysate.



Our Abpromise guarantee covers the use of ab58754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use 20µl for 106 cells. (Good for 10^6 cells in suspension or for 100 µl of whole blood.)

ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similaritiesContains 1 Link domain.
  • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [MEM-263] (Phycoerythrin) images

  • All lanes : Anti-CD44 antibody [MEM-263] (Phycoerythrin) (ab58754) at 2 µg/ml

    Lane 1 : Human peripheral blood lymphocytes
    Lane 2 : Canine peripheral blood lymphocytes
    Lane 3 : Porcine peripheral blood lymphocytes

    anti-mouse IgG/HRP

    Predicted band size : 82 kDa
    Observed band size : 82 kDa

References for Anti-CD44 antibody [MEM-263] (Phycoerythrin) (ab58754)

This product has been referenced in:
  • Linard C  et al. Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs. Stem Cells Transl Med 2:916-27 (2013). Pig . Read more (PubMed: 24068742) »

See 1 Publication for this product

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Thank you for contacting us. We do have products that are conjugated to match your needs, Ab81424 Anti-CD44 antibody [MEM-263] is conjugated with APC. Ab58754 Anti-CD44 antibody [MEM-263] (Phycoerythrin). I've included links to each below: http://www.a...

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