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Tissue/ cell preparation (Human). (Leucocytes of patient suffering from LGL type leukaemia).
ab23777 reacts with the same epitope as Hermes-1 on the molecule, which is a leucocyte adhesion molecule (hyaluronan receptor), participating at homing.
Our Abpromise guarantee covers the use of ab23777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24986866|
|Flow Cyt||Use 5µl for 106 cells.|
Umbilical cord mesenchymal stem cells were treated or not with chondroitinase ABC or Hylase and placed in co-culture with inflammatory cells seeded in a transwell insert with a 0.44-μm pore. Expression and localization of TSG6 and CD44 were evaluated by immunocytochemistry using immunoflurence with ab23777.