Anti-CD44 antibody [MRC OX-49] (Biotin) (ab112179)


  • Product nameAnti-CD44 antibody [MRC OX-49] (Biotin)
    See all CD44 primary antibodies
  • Description
    Mouse monoclonal [MRC OX-49] to CD44 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat T cell blasts.

  • Positive control
    • Rat Thymus, Spleen and Lymph Node cells (Wistar strain).



Our Abpromise guarantee covers the use of ab112179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.5-1µg for 106 cells. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similaritiesContains 1 Link domain.
  • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LHR antibody
    • BA-1 antibody
    • CD 44 antibody
    • CD44 antibody
    • CD44 antigen antibody
    • CD44 molecule (Indian blood group) antibody
    • CD44 molecule antibody
    • CD44_HUMAN antibody
    • CDw44 antibody
    • Cell surface glycoprotein CD44 antibody
    • chondroitin sulfate proteoglycan 8 antibody
    • CSPG8 antibody
    • ECMR-III antibody
    • Epican antibody
    • Extracellular matrix receptor III antibody
    • GP90 lymphocyte homing/adhesion receptor antibody
    • HCELL antibody
    • hematopoietic cell E- and L-selectin ligand antibody
    • Heparan sulfate proteoglycan antibody
    • Hermes antigen antibody
    • homing function and Indian blood group system antibody
    • HSA antibody
    • HUTCH-I antibody
    • HUTCH1 antibody
    • Hyaluronate receptor antibody
    • IN antibody
    • MC56 antibody
    • MDU2 antibody
    • MDU3 antibody
    • MGC10468 antibody
    • MIC4 antibody
    • MUTCH1 antibody
    • PGP-1 antibody
    • PGP-I antibody
    • PGP1 antibody
    • Phagocytic glycoprotein 1 antibody
    • Phagocytic glycoprotein I antibody
    see all

Anti-CD44 antibody [MRC OX-49] (Biotin) images

  • ab112179, at 0.5 µg/106 cells, staining CD44 in Rat Spleen cells (Strain: Wistar) by Flow Cytometry.
    Percentage of cells stained above control (Biotin Mouse IgG2a): 66.2%.

References for Anti-CD44 antibody [MRC OX-49] (Biotin) (ab112179)

ab112179 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We do not have any data regarding the use of this antibody with adherent cells, and whether the use of trypsin affected CD44 detection. However, I have found some information which might be helpful. Please h...

Read More

Thank you for your call yesterday and for your patience while I have looked through the literature regarding your enquiry.

I have found a reference that used the CD44 antibody, clone F10-44-2 to immunoprecipitate HIV-1 particles (see attached...

Read More