The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 5-10µl for 106 cells.
IP: Use at an assay dependent dilution.
May also be used in antibody mediated cytotoxicity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Contains 1 Link domain.
The lectin-like LINK domain is responsible for hyaluronan binding.
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N-glycosylated. O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
hematopoietic cell E- and L-selectin ligand antibody
Heparan sulfate proteoglycan antibody
Hermes antigen antibody
homing function and Indian blood group system antibody
Hyaluronate receptor antibody
INLU-related p80 Glycoprotein antibody
Phagocytic glycoprotein 1 antibody
Phagocytic glycoprotein I antibody
Soluble CD44 antibody
Anti-CD44 antibody [T2-F4] images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [T2-F4] (ab40983)This image is courtesy of an Abreview submitted by Zahra Abdalla
ab40983 staining CD44 in Human OSCC tongue tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum + 0.1% BSA for 30 minutes at 21°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 2 hours at 21°C. An Alexa Fluor®488-conjugated Goat anti-rat IgG monoclonal(1/400) was used as the secondary antibody.
Flow Cytometry - CD44 antibody [T2-F4] (ab40983)This image is courtesy of an anonymous Abreview.
ab40983 staining mouse embryonic stem cells by Flow Cytometery. Cells were fixed in paraformaldehyde and gating was done on live cells. The primary antibody was diluted 1/100 and incubated with sample for 30 minutes at 25°C. A PE conjugated goat polyclonal to rat IgG, diluted 1/100 was used as secondary.
Flow Cytometry - Anti-CD44 antibody [T2-F4] (ab40983)Image courtesy of an anonymous Abreview.
ab40983 used in Flow Cytometry. HUCPVCs were fixed in paraformaldehyde.ab40983 used at a 1/100 dilution. Secondary used was a goat anti-rat polyclonal conjugated to PE, used at a 1/100 dilution. Purple plot shows rat IgG isotype control and secondary antibodyGreen plot shows ab40983 and secondary antibody
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [T2-F4] (ab40983)This image is courtesy of an anonymous abreviewer.
ab40983 staining CD44 protein on mutant mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rat IgG, diluted 1/200, was used as the secondary antibody.