The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl (at a concentration of 10 µg/ml) for 106 cells in 100µl.
IHC-Fr: Use at a concentration of 10 µg/ml.
IHC-P: Use at a concentration of 10 µg/ml. Antigen retrieval using heat treatment prior to staining of paraffin sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. There are 17 different isoforms.
References for Anti-CD44v3 antibody [VFF-327] (ab34229)
This product has been referenced in:
Alam TN et al. Differential expression of CD44 during human prostate epithelial cell differentiation. J Histochem Cytochem52:1083-90 (2004).
Read more (PubMed: 15258184) »
Seiter S et al. Mitigation of delayed-type hypersensitivity reactions by a CD44 variant isoform v3-specific antibody: blockade of leukocyte egress. J Invest Dermatol113:11-21 (1999).
Read more (PubMed: 10417612) »