The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
1/400 - 1/500.
Use at an assay dependent concentration.
FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
Sequence similaritiesContains 1 Link domain.
DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
Post-translational modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
Cellular localizationMembrane. Colocalizes with actin in membrane protrusions at wounding edges.
Immunoperoxidase staining of formalin fixed, paraffin embedded human breast cancer tissue with ab30436 at 1/200 dilution.
References for Anti-CD44v6 antibody [VFF-7] (ab30436)
This product has been referenced in:
Rezniczek GA et al. Dynamic changes of tumor gene expression during repeated pressurized intraperitoneal aerosol chemotherapy (PIPAC) in women with peritoneal cancer. BMC Cancer16:654 (2016).
Read more (PubMed: 27542596) »
Liebertz DJ et al. Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1. Head Neck Oncol2:5 (2010).
Read more (PubMed: 20175927) »