Overview

  • Product nameCD44var (v6) Human ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.05 ng/ml
  • Range
    0.32 ng/ml - 10 ng/ml
  • Recovery

    106 %

    Sample specific recovery
    Sample type Average % Range
    Serum 106 89% - 115%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD44var (v6) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human CD44var (v6) concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), and urine.

    CD44var (v6) specific antibodies have been precoated onto a 96-well plate. Standards and test samples are added to the wells and along with a CD44var (v6) HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD44var (v6) amount of sample captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 2 units
    Blue-Dye 1 x 400µl
    Green-Dye 1 x 400µl
    HRP-Conjugate anti-Human CD44var (v6) monoclonal antibody 2 x 10µl
    Human CD44var (v6) Standard Lyophilized 20 ng/mL upon reconstitution) 2 vials
    Microplate coated with anti CD44var (v6) monoclonal antibody (12 x 8 wells) 1 unit
    Sample Diluent 1 x 50ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • Research Areas
  • FunctionReceptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
  • Tissue specificityIsoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
  • Sequence similaritiesContains 1 Link domain.
  • DomainThe lectin-like LINK domain is responsible for hyaluronan binding.
  • Post-translational
    modifications
    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Alternative names
    • CD44
    • CD44 antigen
    • CD44 molecule
    • CD44_HUMAN
    • CDw44
    • Cell surface glycoprotein CD44
    • Chondroitin sulfate proteoglycan 8
    • CSPG8
    • ECMR-III
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • Hematopoietic cell E and L selectin ligand
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • Homing function and Indian blood group system
    • HUTCH-I
    • Hyaluronate receptor
    • IN
    • Indian blood group
    • LHR
    • MC56
    • MDU2
    • MDU3
    • MIC4
    • PGP-1
    • PGP-I
    • Pgp1
    • Phagocytic glycoprotein 1
    • Phagocytic glycoprotein I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD44var (v6) Human ELISA kit images

  • Representative Standard Curve using ab119519.

Protocols

References for CD44var (v6) Human ELISA kit (ab119519)

ab119519 has not yet been referenced specifically in any publications.

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After reading the protocol I am sure that the whole plate is coated with the antibody as all the reagents are designed to be sufficient for 12 stripes. As mentioned in the protocol, the 32 wells are just an examp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"