• Product nameAnti-CD45 antibody [0.N.125]
    See all CD45 primary antibodies
  • Description
    Mouse monoclonal [0.N.125] to CD45
  • SpecificityThis antibody recognizes the CD45 leucocyte common antigen (LCA) family.
  • Tested applicationsSuitable for: ICC/IF, ELISA, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rhesus monkey
  • Immunogen

    Tissue/ cell preparation (Human): Non T, non B ALL cell line (REH).


Our Abpromise guarantee covers the use of ab33533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. See Abreview.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at 2 µg/mg of lysate.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 147 kDa.


  • FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe first PTPase domain interacts with SKAP1.
  • Post-translational
    Heavily N- and O-glycosylated.
  • Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [0.N.125] images

  • ab33533 staining human blood mononuclear cells by ICC/IF.  Cells were PFA fixed and blocked in 2.5% BSA for 30 minutes at 20°C prior to incubation with the primary antibody, diluted 1/100, for 18 hours at 4°C.  A FITC conjugated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

References for Anti-CD45 antibody [0.N.125] (ab33533)

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). IF ; Human . Read more (PubMed: 26732624) »
  • Clayton KL  et al. T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases. J Immunol 192:782-91 (2014). Human . Read more (PubMed: 24337741) »

See all 4 Publications for this product

Product Wall

The immunogen for this antibody is:

Non-T, non-B Acute lymphoblastic leukemia cell line (identified as REH cell line). CD45 is a transmembrane protein, that is also identified as Protein tyrosine phosphatase, receptor type, C (PTPRC). Multip...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human blood MNC)
Specification Human blood MNC
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 31 2008