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The details of the immunogen for this antibody are not available.
The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation - this antibody can help act as a marker for determining the origin of host and donor cells. This antibody has also been shown to be useful for identifying the allotype of leucocytes in transgenic and "knockout" mice.
Our Abpromise guarantee covers the use of ab25463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.3µg for 106 cells. ab103534-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab25463 has not yet been referenced specifically in any publications.