Specificityab25350 recognises CD45.2: it recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).
The details of the immunogen for this antibody are not available.
General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Storage instructionsShipped at 4°C. Store at +4°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 0.3µg for 106 cells.
IP: Use at an assay dependent dilution.
Note: ab25350 can be used to distinguish markers for origin of donor and host cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.
DomainThe first PTPase domain interacts with SKAP1.
Post-translational modificationsHeavily N- and O-glycosylated.
Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
Protein tyrosine phosphatase receptor type c polypeptide antibody
Protein tyrosine phosphatase, receptor type C antibody
protein tyrosine phosphatase, receptor type, C antibody
Protein tyrosine phosphatase, receptor type, c polypeptide antibody
Receptor-type tyrosine-protein phosphatase C antibody
T200 glycoprotein antibody
T200 leukocyte common antigen antibody
Anti-CD45 antibody  (Cy5 ®) images
Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody  (Cy5 ®) (ab25350)This image is courtesy of an Abreview submitted by Eike Wegener
ab25350 staining CD45 in Mouse spleen cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Target Retrieval Solution pH6.1 and blocked with Rodent Block M for 30 minutes at 25°C. Samples were incubated with primary antibody (1/50 in Antibody Dilutent) for 12 hours at 4°C.