Anti-CD45 antibody [104] (Cy5 ®) (ab25350)

Overview

  • Product name
    Anti-CD45 antibody [104] (Cy5 ®)
    See all CD45 primary antibodies
  • Description
    Mouse monoclonal [104] to CD45 (Cy5 ®)
  • Conjugation
    Cy5 ®. Ex: 650nm, Em: 667nm
  • Specificity
    ab25350 recognises CD45.2: it recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).
  • Tested applications
    Suitable for: Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Applications

Our Abpromise guarantee covers the use of ab25350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
ICC/IF
  • Application notes
    Flow Cyt: Use 0.3µg for 106 cells.
    IP: Use at an assay dependent dilution.

    Note: ab25350 can be used to distinguish markers for origin of donor and host cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
    • Involvement in disease
      Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
      Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain
      The first PTPase domain interacts with SKAP1.
    • Post-translational
      modifications
      Heavily N- and O-glycosylated.
    • Cellular localization
      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • B220 antibody
      • CD 45 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lyt-4 antibody
      • OTTHUMP00000033813 antibody
      • OTTHUMP00000033816 antibody
      • OTTHUMP00000033817 antibody
      • OTTHUMP00000038574 antibody
      • Protein tyrosine phosphatase receptor type c polypeptide antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Anti-CD45 antibody [104] (Cy5 ®) images

    • ab25350 staining CD45 in Mouse spleen cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Target Retrieval Solution pH6.1 and blocked with Rodent Block M for 30 minutes at 25°C. Samples were incubated with primary antibody (1/50 in Antibody Dilutent) for 12 hours at 4°C.

      See Abreview

    References for Anti-CD45 antibody [104] (Cy5 ®) (ab25350)

    ab25350 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Rodent Block M (Biocare Medical) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Sample
    Mouse Cell (Spleen)
    Specification
    Spleen
    Permeabilization
    Yes - Target Retrieval Solution pH6.1 (DAKO)
    Fixative
    Paraformaldehyde
    Username

    Mr. Eike Wegener

    Verified customer

    Submitted Sep 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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