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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.2µg for 106 cells. ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD45 with ab25603 at 0.1 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG2b-PE.
Flow cytometry analysis of DAPI stained alive murine cells retrivived via a bronchoaveolar lavage using PBS-EDTA 3mM, labelling CD45 with ab25603 at 1/3000 incubated for 20 minutes at 20°C in PBS-EDTA 3mM, with a primary conjugate against CD45-APC and DAPI. There was no secondary used as ab25603 is a phycoerythrin conjugate. Cells were gated on alive DAPI stained CD45+ cells. Grey histogram represents isotype control and CD45 histogram is in purple. The positive labelling of 100% CD45 + cells preselected on APC detector, confirms the efficacy of the antibody to detect CD45 antigen.