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The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab25603 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.2µg for 106 cells. ab136585-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
Flow Cytometry analysis of BALB/c mouse splenocytes labeling CD45 with ab25603 at 0.1 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG2b-PE.
Flow cytometry analysis of DAPI stained alive murine cells retrivived via a bronchoaveolar lavage using PBS-EDTA 3mM, labelling CD45 with ab25603 at 1/3000 incubated for 20 minutes at 20°C in PBS-EDTA 3mM, with a primary conjugate against CD45-APC and DAPI. There was no secondary used as ab25603 is a phycoerythrin conjugate. Cells were gated on alive DAPI stained CD45+ cells. Grey histogram represents isotype control and CD45 histogram is in purple. The positive labelling of 100% CD45 + cells preselected on APC detector, confirms the efficacy of the antibody to detect CD45 antigen.