Anti-CD45 antibody [I3/2.3] (Phycoerythrin -Texas Red®) (ab51482)

Overview

  • Product nameAnti-CD45 antibody [I3/2.3] (Phycoerythrin -Texas Red®)
    See all CD45 primary antibodies
  • Description
    Rat monoclonal [I3/2.3] to CD45 (Phycoerythrin -Texas Red®)
  • ConjugationPhycoerythrin -Texas Red®. Ex: 496nm, Em: 613nm
  • Specificityab51482 is specific to CD45 (T200 or leukocyte common antigen/LCA, all isoforms).
  • Tested applicationsSuitable for: RIA, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

Applications

Our Abpromise guarantee covers the use of ab51482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration. Quantification of CD45 molecules on cell surfaces.
Flow Cyt Use 0.2µg for 106 cells. ab98984-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications
    Heavily N- and O-glycosylated.
  • Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45 antibody [I3/2.3] (Phycoerythrin -Texas Red®) images

  • ab51482 staining CD45 in Mouse speen cells by Flow Cytometry.  The splenocytes were dispersed with 70µm strainer and erythrolyzed in PBS. The sample was incubated with the primary antibody (undiluted) for 30 minutes at 0°C.

    Gateing Strategy: FSC, SSC.

     

    See Abreview

  • ab51482 staining the CD45 in Mouse blood by Flow Cytometry. Cells were prepared in EDTA blood and the sample buffer was EDTA buffer. The sample was incubated with the primary antibody (1/100 in PBS) for 30 minutes at 20°C.
    Gating Strategy: FR - SS.

    See Abreview

References for Anti-CD45 antibody [I3/2.3] (Phycoerythrin -Texas Red®) (ab51482)

This product has been referenced in:
  • Oerlemans MI  et al. Active Wnt signaling in response to cardiac injury. Basic Res Cardiol 105:631-41 (2010). Flow Cyt . Read more (PubMed: 20373104) »
  • Haidl ID  et al. Detection of restricted isoform expression and tyrosine phosphatase activity of CD45 in murine dendritic cells. Eur J Immunol 25:3370-4 (1995). Read more (PubMed: 8566025) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (spleen)
Specification spleen
Preparation Cell harvesting/tissue preparation method: splenocytes dispersed with 70µm strainer and erythrolyzed
Sample buffer: PBS
Permeabilization No
Gating Strategy FSC, SSC
Username

Abcam user community

Verified customer

Submitted Mar 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (blood)
Specification blood
Preparation Cell harvesting/tissue preparation method: EDTA bood
Sample buffer: EDTA buffer
Fixation optilyse
Permeabilization No
Gating Strategy FS - SS
Username

Abcam user community

Verified customer

Submitted Mar 12 2013

Thank you for contacting us. I am sorry this vial did not contain the correct amount of antibody. As requested, I have issued a free of charge replacement with the order number ***.

To check the status of the order please contact our Custome...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"