The details of the immunogen for this antibody are not available.
Our Abpromise guarantee covers the use of ab51482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|RIA||Use at an assay dependent concentration. Quantification of CD45 molecules on cell surfaces.|
|Flow Cyt||Use 0.2µg for 106 cells. ab98984-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
ab51482 staining CD45 in Mouse speen cells by Flow Cytometry. The splenocytes were dispersed with 70µm strainer and erythrolyzed in PBS. The sample was incubated with the primary antibody (undiluted) for 30 minutes at 0°C.
Gateing Strategy: FSC, SSC.