CD45 antibody [IH-1] (FITC) (ab28085)

Product Name 

CD45 antibody [IH-1] (FITC)

Product type 

Primary antibodies

Description 

Mouse monoclonal [IH-1] to CD45 (FITC)

Immunogen 

The details of the immunogen for this antibody are not available.

Reacts with 
(species key)

Gpig

Specificity 

ab28085 recognises CD45 on all leucocytes.

Tested applications 
(see key)

Flow Cyt

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use working dilution from neat to 1/10. Use 10µl for 10⁶ cells in 100ul.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Dendritic Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> NK Cell Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Neuroscience >> Cell Type Marker >> Glia marker >> Microglia marker
Signal Transduction >> Protein Phosphorylation >> Tyrosine Phosphatases
Immunology >> Cell Type Markers >> CD >> Non-lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD

Relevance 

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

IH-1

Myeloma 

NS1

Isotype 

IgG1

Purity 

Protein A purified

Conjugation notes 

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC)

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: 1% BSA, PBS, pH 8.0

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.

References for CD45 antibody [IH-1] (FITC) (ab28085)

  This clone has been used in:

Hart IJ et al. Subpopulations of guinea-pig T lymphocytes defined by isoforms of the leucocyte common antigen. Immunology 77:377-84 (1992). PubMed: 1362184

If you publish research using ab28085 please let us know so that we can cite the reference on this datasheet.

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