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Thymocytes and T lymphocytes. (Human)
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Our Abpromise guarantee covers the use of ab28106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC||Use at an assay dependent concentration.|
ab28106 staining the CD45 in Human PBMCs by Flow Cytometry. Cells were prepared by Ficoll-Hypaque isolation of PBMCs from buffy coat. The sample was incubated with the primary antibody (1/25 in PBS + 2% Human serum and 1mM EDTA) for 25 minutes at 4°C.