Overview

  • Product nameAnti-CD45 antibody [MRC OX-1]
    See all CD45 primary antibodies
  • Description
    Mouse monoclonal [MRC OX-1] to CD45
  • Specificityab33923 recognises CD45, also known as the leucocyte common antigen (LCA).
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins.

Applications

Our Abpromise guarantee covers the use of ab33923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/100. Use 10ul of the suggested working dilution to label 106 cells in 100ul.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.
  • Involvement in diseaseDefects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • DomainThe first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications
    Heavily N- and O-glycosylated.
  • Cellular localizationMembrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • B220 antibody
    • CD 45 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lyt-4 antibody
    • OTTHUMP00000033813 antibody
    • OTTHUMP00000033816 antibody
    • OTTHUMP00000033817 antibody
    • OTTHUMP00000038574 antibody
    • Protein tyrosine phosphatase receptor type c polypeptide antibody
    • protein tyrosine phosphatase, receptor type, C antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

References for Anti-CD45 antibody [MRC OX-1] (ab33923)

This product has been referenced in:
  • Ortiz-Masiá D  et al. iNOS-derived nitric oxide mediates the increase in TFF2 expression associated with gastric damage: role of HIF-1. FASEB J 24:136-45 (2010). IHC-P ; Rat . Read more (PubMed: 19741170) »
  • Vansthertem D  et al. Label-retaining cells and tubular regeneration in postischaemic kidney. Nephrol Dial Transplant : (2008). Read more (PubMed: 18678561) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (head and neck cancer)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization No
Specification head and neck cancer
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 19 2016

Vielen Dank für Ihre Antwort.

Die Referenznummer Ihrer Gutschrift ist 123456.

Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, ...

Read More

Vielen Dank für Ihre Geduld.

Es ist tatsächlich so, dass wir diesen Antikörper unter verschiedenen Bedingungen nicht in der IHC-P zum Laufen gebracht habe. Zwar haben wir die Publikation in welcher er in IHC-P verwendet wird, aber wir haben ...

Read More

Mir ist aufgefallen, dass internen Daten bezüglich der Reaktivität dieses Antikörpers in der IHC-P widersprüchlich sind: Angeblich funktionierte dieser Antikörper in unseren Händen nicht in der IHC-P. Diese Applikation wurde später automatisch durch un...

Read More

Thank you for your reply.

Your credit note ID is 00000.

This can be used for the bill you received for this antibody. Please let our finance department (mailto:creditcontrol@abcam.com) know how you would like to use it.

...

Read More

Thank you for contacting us and sorry for the delay in getting back to you.

The anti-CD45 antibody(ab33923) has been used with rat samples with paraffin embedded tissue sections. Please refer to the reference below:

Ortiz-Masi&aacut...

Read More

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"